|
KMID : 0880520110470030155
|
|
Chonnam Medical Journal
2011 Volume.47 No. 3 p.155 ~ p.159
|
|
|
Effects of Cyclic Nucleotide-Gated Channels in Vestibular Nuclear Neurons
|
|
Heo Sung-Hyuk
Jang Su-Jeong
Jeong Han-Seong
Park Jong-Seong
|
|
|
Abstract
|
|
|
|
This study was designed to investigate the effects an 8-Br-cGMP on the neuronal activity of rat vestibular nuclear cells. Sprague-Dawley rats aged 14 to 16 days were decapitated under ether anesthesia. After treatment with pronase and thermolysin, the dissociated vestibular nuclear cells were transferred into a chamber on an inverted microscope. Spontaneous action potentials and potassium currents were recorded by standard patch-clamp techniques under current and voltage-clamp modes. Twelve vestibular nuclear cells revealed excitatory responses to 1-5 μM of 8-Br-cGMP, and 3 neurons did not respond to 8-Br-cGMP. Whole potassium currents of vestibular nuclear cells were decreased by 8-Br-cGMP (n=12). After calcium-dependent potassium currents were blocked by tetraethylammonium, the potassium currents were not decreased by 8-Br-cGMP. These experimental results suggest that 8-Br-cGMP changes the neuronal activity of vestibular nuclear cells by blocking the calcium-dependent potassium currents that underlie the afterhyperpolarization.
|
|
|
KEYWORD
|
|
|
Nucleotides, Cyclic, Vestibule, Neurons
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|